A cylindrical specimen holder for electron cryo-tomography
نویسندگان
چکیده
منابع مشابه
A scanning electron microscopy specimen holder for viewing different angles of a single specimen.
The specimen holder for scanning electron microscopy described herein allows a single specimen to be examined in any possible view and significantly improves object illumination. The specimen is glued to a fine pin and flexibly mounted on a double-sided adhesive conductive pad on a rotatable pivot. A milled pot placed beneath the specimen acts as an electron trap. This provides a homogeneous bl...
متن کاملLocalize.pytom: a modern webserver for cryo-electron tomography
Localize.pytom, available through http://localize.pytom.org is a webserver for the localize module in the PyTom package. It is a free website and open to all users and there is no login requirement. The server accepts tomograms as they are imaged and reconstructed by Cryo-Electron Tomography (CET) and returns densities and coordinates of candidate-macromolecules in the tomogram. Localization of...
متن کاملA Set of Collets for Manipulating the Specimen Holder of the Siemens Electron Microscope
The modification of the Siemens electron microscope grid-holder suggested by Elbers (1) among its other advantages reduces contamination by reducing the handling of the specimen holder either with the fingers or using paper tissue or other fabrics. By using the set of collets described here such handling is entirely eliminated although the operations involved in changing the specimen may still ...
متن کاملAn in situ nanoindentation specimen holder for a high voltage transmission electron microscope.
We describe in detail, the design, construction, and testing of a specimen holder that allows for the nanoindentation of surfaces while viewing in cross-section in a high voltage transmission electron microscope (TEM). This nanoindentation specimen holder, having three-axis position control of a diamond indenter in combination with micromachined specimens, allows for the first time the dynamic ...
متن کاملCryo-electron tomography of rubella virus.
Rubella virus is the only member of the Rubivirus genus within the Togaviridae family and is the causative agent of the childhood disease known as rubella or German measles. Here, we report the use of cryo-electron tomography to examine the three-dimensional structure of rubella virions and compare their structure to that of Ross River virus, a togavirus belonging the genus Alphavirus. The ecto...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Ultramicroscopy
سال: 2014
ISSN: 0304-3991
DOI: 10.1016/j.ultramic.2013.10.016